MersV1, Main, Exploration, bibRecord, 003B57

Immune Responses but No Protection against SHIV by Gene-Gun Delivery of HIV-1 DNA Followed by Recombinant Subunit Protein Boosts

Identifieur interne : 003B57 ( Main/Exploration ); précédent : 003B56; suivant : 003B58

Immune Responses but No Protection against SHIV by Gene-Gun Delivery of HIV-1 DNA Followed by Recombinant Subunit Protein Boosts

Auteurs : Per Putkonen [Suède] ; Marlene Quesada-Rolander [Suède] ; Ann-Charlotte Leandersson [Suède] ; Stefan Schwartz [Suède] ; Rigmor Thorstensson [Suède] ; Kenji Okuda [Japon] ; Britta Wahren [Suède] ; Jorma Hinkula [Suède]

Source :

Virology [ 0042-6822 ] ; 1998.

RBID : ISTEX:EBB41500BFE3B79FBCA82D48F879725EF427A90B

English descriptors

Teeft :
- Antibody responses, Assay, Biberfeld, Control animals, Control monkeys, Cynomolgus, Cynomolgus monkeys, Dos, Hinkula, Humoral, Immune, Immune defic, Immune responses, Immunization, Immunodeficiency, Letvin, Lymphoid cells, Macaque, Monkey, Mucosal, Neutralizing, Neutralizing antibodies, Peptide, Peripheral blood cells, Plasmid, Protective immunity, Protein boosts, Protein immunizations, Putkonen, Recombinant, Recombinant proteins, Regulatory genes, Regulatory proteins, Ribi adjuvant, Shiv, Shiv challenge, Simian immunodeficiency virus, Subunit, Synthetic peptides, Thorstensson, Triplicate wells, Vaccinated, Vaccinated animals, Vaccinated monkeys, Vaccination, Vaccine, Vaccinee, Viral, Viral load, Virion, Virol, Wahren.

Abstract

Abstract: The efficacy of combining immunization with human immunodeficiency vitus type 1 (HIV-1) DNA and HIV-1 recombinant proteins to obtain protection from chimeric simian/human immunodeficiency virus (SHIV) was determined. Four cynomolgus monkeys received four gene-gun immunizations intraepidermally of plasmid DNA encoding HIV-1laienv(gp160),gag, tat, nef,andrevproteins. Ten micrograms of DNA was used per immunization. The animals were boosted twice intramuscularly with 50 μg of HIV-1laiEnv (MicroGeneSys), Gag, Tat, Nef, and Rev recombinant proteins mixed in Ribi adjuvant. The antibody responses were amplified following the administration of the recombinant subunit boosts. One month after the final subunit immunization, the vaccinated animals together with four control animals were challenged intravenously with 10 monkey infectious doses of SHIV that expresses theenv, tatandrevgenes of HIV-1 and gag and nef from SIV. However, only low titers of neutralizing antibodies were present at the day of challenge. The consecutive HIV-1 DNA and recombinant protein immunizations induced B- and T-cell responses but not protection against SHIV replication nor reduction of the viral load.

Url:

https://api.istex.fr/ark:/67375/6H6-7QJ7MQHR-D/fulltext.pdf

DOI: 10.1006/viro.1998.9379

Affiliations:

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Le document en format XML

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<term>Cynomolgus monkeys</term>
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<term>Vaccinated monkeys</term>
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<front><div type="abstract" xml:lang="en">Abstract: The efficacy of combining immunization with human immunodeficiency vitus type 1 (HIV-1) DNA and HIV-1 recombinant proteins to obtain protection from chimeric simian/human immunodeficiency virus (SHIV) was determined. Four cynomolgus monkeys received four gene-gun immunizations intraepidermally of plasmid DNA encoding HIV-1laienv(gp160),gag, tat, nef,andrevproteins. Ten micrograms of DNA was used per immunization. The animals were boosted twice intramuscularly with 50 μg of HIV-1laiEnv (MicroGeneSys), Gag, Tat, Nef, and Rev recombinant proteins mixed in Ribi adjuvant. The antibody responses were amplified following the administration of the recombinant subunit boosts. One month after the final subunit immunization, the vaccinated animals together with four control animals were challenged intravenously with 10 monkey infectious doses of SHIV that expresses theenv, tatandrevgenes of HIV-1 and gag and nef from SIV. However, only low titers of neutralizing antibodies were present at the day of challenge. The consecutive HIV-1 DNA and recombinant protein immunizations induced B- and T-cell responses but not protection against SHIV replication nor reduction of the viral load.</div>
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Immune Responses but No Protection against SHIV by Gene-Gun Delivery of HIV-1 DNA Followed by Recombinant Subunit Protein Boosts

Immune Responses but No Protection against SHIV by Gene-Gun Delivery of HIV-1 DNA Followed by Recombinant Subunit Protein Boosts

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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